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RPMI-1640 Medium-Biosci™ Dakewe

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SKU: mode_7587227304117
Price: 11.40 USD
Availability: InStock

Original price was: $ 19,00.Current price is: $ 11,40.

Size

Clear

SKU: mode_7587227304117 Category: Uncategorized Tag: rpmi-1640-medium

Note:Suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas, require supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Product Description

RPMI-1640 Medium was developed by Moore et al., at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI-1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas. RPMI-1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Specifications

Volume	500mL
Supplementation	Need additional
Glutamine	L-Glutamine
Phenol Red	Phenol Red
Antibiotics	No Antibiotics
Sodium Pyruvate	No Sodium Pyruvate
Sodium Bicarbonate	Sodium Bicarbonate
HEPES Buffer	No HEPES
pH	7.0-7.6
Osmolality	265~305 mOsmol/kg
Endotoxin	<0.3 EU/mL

Formulation

Components (mg/L)

Amino Acids

Glycine	10
L-Arginine (free base)	200
L-Asparagine (anhyd.)	50
L-Aspartic Acid	20
L-Cystine•2HCl	65
L-Glutamic Acid	20
L-Glutamine	300
L-Histidine (free base)	15
L-Hydroxyproline	20
L-Isoleucine	50
L-Leucine	50
L-Lysine•HCl	40
L-Methionine	15
L-Phenylalanine	15
L-Proline	20
L-Serine	30
L-Threonine	20
L-Tryptophan	5
L-Tyrosine•2Na•2H₂O	29
L-Valine	20

Vitamins

D-Biotin	0.2
Choline chloride	3
Folic Acid	1
myo-Inositol	35
Niacinamide	1
p-Amino Benzoic Acid	1
D-Pantothenic Acid•1/2 Ca	0.25
Pyridoxal•HCl	1
Riboflavin	0.2
Thiamine•HCl	1
Vitamin B-12	0.005

Inorganic Salts

Ca(NO₃)₂•4H₂O	100
MgSO₄(anhyd.)	48.84
KCl	400
NaHCO₃	2000
NaCl	6000
NaH₂PO₄(anhyd.)	800

Other

D-Glucose	2000
Glutathione (reduced)	1
Phenol Red•Na	5

Storage

Stored at 2℃~8℃, away from light, the shelf life is 12 months.

Notes

Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.

Document

RPMI-1640 Medium-Biosci™

Size	500mL/Bottle

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Returns

The stock market is always changing. It is not managed entirely by us, as we're involved with several parties such as the factory and our storage. The levels of stock can change at any given time. Be aware that it is possible for your order to run out of stock even after you have placed the order.

The policy is 30 days. However, if 30 days have passed since you purchased your item, we will not be able to offer you a return or exchange.

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