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Human IFN-γ Precoated ELISA kit-96T-BioSci™ Dakewe

Name: Now you can browse the latest collections from Human IFN-γ Precoated ELISA kit-96T-BioSci™ Dakewe
SKU: mode_7461960220853
Price: 120.00 USD
Availability: InStock

Original price was: $ 300,00.Current price is: $ 120,00.

Size

Clear

SKU: mode_7461960220853 Category: Uncategorized Tags: Elisa, Kit

Product Description

The human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by “sandwich one step”. The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.

Product Details

Detection range : 12.5-400 pg/mL

Sensitivity: 5 pg/mL

Precision : Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%

Stored : 2 – 8 °C

Verified Reactivity：Human

Reagents Provided and Reconstitution

Reagents	Quantity	Reconstitution
Cytokine standard	2 vials	Lyophilized, reconstitute with the volume of reagent indicated on the vial
Biotinylated antibody	2 vials	Dilute 50 times in Diluent buffer（1×）
Streptavidin-HRP	2 vials	Dilute 100 times in Diluent buffer（1×）
Diluent buffer（1×）	3 vials	Ready-to-use
Washing buffer（50×）	1 vial	Dilute 50 times in distilled Water
TMB	1 vial	Ready-to-use
Stop solution	1 vial	Ready-to-use
Precoated ELISA plates	8×12	Ready-to-use
Plastic cover	4 sheets	Ready-to-use

Material Required but not Provided

96-well ELISA plate (microplate spectrophotometer).
Pipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.
Distilled water, fresh absorbent paper.
Vortex mixer and magnetic stirrer

Scientific Data

Assay Procedure Summary

Add 100 μL of sample, standard or control per well
Add 50 μL of diluted Biotinylated Detection antibody to all wells
Incubate 2 hours at room temperature
Wash three times
Add 100 μL of streptavidin-HRP to all wells
Incubate 20 minutes at room temperature
Wash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.
Add 100 μL Stop solution to all wells
Read at 450 nm within 15 minutes

Document

Technical Data Sheet

Size	96T

Quick Shipping and Professional Packaging

We can offer a variety of shipping options due to our long-term relationship with UPS FedEx DHL. Our warehouse staff will package each item according to our strict specifications. Your products will be subjected to a thorough examination and will be safely packaged prior to being shipped. Everyday we deliver thousands of packages to clients across many countries. This is a sign of our determination to become the biggest online retailer in the world. There are distribution centers and warehouses in Europe as well as the USA.

Orders that contain more than 1 item are given processing time for each item.

Before shipment, all ordered items will be thoroughly inspected. Most orders are shipped within 48-hours. Delivery is expected to take between 3 and 7 days.

Returns

The stock market is always changing. It is not managed entirely by us, as we're involved with several parties such as the factory and our storage. The levels of stock can change at any given time. Be aware that it is possible for your order to run out of stock even after you have placed the order.

The policy is 30 days. However, if 30 days have passed since you purchased your item, we will not be able to offer you a return or exchange.

To be eligible for a refund the item must be in good condition and in the same state as when you received it. It should also be in the original package.