Intended Use
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The 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets.
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The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.
Product Characteristics
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The kit is designed for multiplex RT-PCR with TaqMan probes.
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For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
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The RTase is easily heat-inactivated at ≥90°C for 1min.
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The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of reaction.
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The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
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The kit also contains Super Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.
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Up to four pairs of gene-specific primers can be applied in one reaction.
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The concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).
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The preferable PCR product size is ≤150bp.
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The kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.
Documents
User Manual
| Size | 2×1ml, 5×1ml, 10ml |
|---|---|
| Style | No ROX, Low ROX, High ROX |
Quick Shipping and Professional Packaging
We can offer a variety of shipping options due to our long-term relationship with UPS FedEx DHL. Our warehouse staff will package each item according to our strict specifications. Your products will be subjected to a thorough examination and will be safely packaged prior to being shipped. Everyday we deliver thousands of packages to clients across many countries. This is a sign of our determination to become the biggest online retailer in the world. There are distribution centers and warehouses in Europe as well as the USA.
Orders that contain more than 1 item are given processing time for each item.
Before shipment, all ordered items will be thoroughly inspected. Most orders are shipped within 48-hours. Delivery is expected to take between 3 and 7 days.
Returns
The stock market is always changing. It is not managed entirely by us, as we're involved with several parties such as the factory and our storage. The levels of stock can change at any given time. Be aware that it is possible for your order to run out of stock even after you have placed the order.
The policy is 30 days. However, if 30 days have passed since you purchased your item, we will not be able to offer you a return or exchange.
To be eligible for a refund the item must be in good condition and in the same state as when you received it. It should also be in the original package.
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