CircRNA Standard Series GENESEED

Product Description:

Geneseed Biotech’s newly optimized circRNA standard series are preparing by   the circPrecise™ platform which has independent intellectual property. The circPrecise™ is based on an innovative upgrade of type I intron self-splicing (PIE) technology, utilizing RNA’s own secondary structure to design an optimal circularization site without introducing exon sequences, and enables the precise preparation for No Scar. The preparation and translation efficiency were greatly improved by coupled transcription-circularization. The circRNA standard series can be used as a positive control for experimental exploration in translation expression validation, transfection delivery evaluation, process optimization, etc.

Circ-eGFP, a circRNA encoding enhanced green fluorescent protein gene (eGFP). After translation and expression, green fluorescence can be observed by fluorescence microscopy.

Circ-mCherry, a circRNA encoding the red fluorescent protein gene. After translation expression, red fluorescence can be observed by fluorescence microscopy.

Circ-Fluc, a circRNA encoding Firefly Luciferase gene. After translation and expression, firefly luciferase activity can be detected experimentally.

 

Product Features:

High integrity

 

Fig.1: After purification, the integrity (CE) of circRNA >95%.

 

High purity

 

Fig.2: After purification, the purity (HPLC) of circRNA >90%.

 

High quality

 

Fig.3: Fluorescence detection of 293T cells transfected with GSPure® Circ-eGFP. Magnification: 100×.

Fig.4: Fluorescence detection of 293T cells transfected with GSPure^® Circ-mCherry. Magnification: 100×.

 

 

 

Fig.5: Luciferase assay results after transfection of 293T cells with GSPure® Circ-Fluc.

 

Kit Contents:

Category	Product Name	Model	Specification
Reporter gene series	GSPure® Circ-eGFP	R0701/R0702/R0703/R0704	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-Fluc	R0801/R0802/R0803/R0804	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-mCherry	R0901/R0902/R0903/R0904	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-beta gal	R1401/R1402/R1403/R1404	100 μg / 500 μg / 1 mg / 10 mg
Tumor antigen series	GSPure® Circ-OVA	R1501/R1502/R1503/R1504	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-HER2	R1601/R1602/R1603/R1604	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-p53	R1701/R1702/R1703/R1704	100 μg / 500 μg / 1 mg / 10 mg
	GSPure® Circ-PD1	R1801/R1802/R1803/R1804	100 μg / 500 μg / 1 mg / 10 mg
Cytokine series	GSPure® Circ-IL2	R1901/R1902/R1903/R1904	100 μg / 500 μg / 1 mg / 10 mg
Gene editing series	GSPure® Circ-Cas9	R2001/R2002/R2003/R2004	100 μg / 500 μg / 1 mg / 10 mg
Protein/antibody substitution series	GSPure® Circ-EPO	R2101/R2102/R2103/R2104	100 μg / 500 μg / 1 mg / 10 mg
iPSC reprogramming series	GSPure® Circ-SOX2	R2201/R2202/R2203/R2204	100 μg / 500 μg / 1 mg / 10 mg

 

Storage and Handling: 

Store the kit at -80 °C. The kit is stable for at least one year from date of receipt.