Total RNA Isolation Kit (For Cells) – Foregene Foregene

Highlights

• The whole process is operated at room temperature (15-25°C), without ice bath and low temperature centrifugation.

• Fast speed: Easy to operate and can be completed in 11 minutes.

• Double column: Rapid column based purification of total RNA, which has both DNA-cleaning Column and RNA-Only column.

• DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase. RNA-only Column and unique formula can efficiently purify RNA.

• Safety: No hazardous organic solvents is required such as Trizol. 

• High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments. The whole kit is RNase-Free, no need to worry about RNA degradation.

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Kit components

• Buffer PRL1, Buffer PRL2
• Buffer PRW1, Buffer PRW2
• RNase-Free ddH2O, DNA-Cleaning Column
• RNA-Only Column
• Instructions

*Please wear gloves and take protective measures during the operation as Buffer cRL1 and Buffer RW1 contain irritation chaotropic salts.

 Work Flow

Diagram

The agarose gel battery diagram of Cell Total RNA Isolation Kit treated the above different numbers of cells,20μl volume elution, take 2μl purified total RNA 1%.

Storage and Shelf Life

• All procedures are carried out at room temperature (15-25℃), including centrifugation. Do not use ice bath or centrifuge at low temperature (4℃).
• The sample should avoid repeated freezing and thawing, otherwise it will lead to the degradation of the extracted RNA and the yield of RNA will also decrease.
• The yield and quality of RNA is tightly related to sample size and volume of elution. For every 500uL Buffer cRL1, the recommended maximum cell volume is 10^6.
• Before using the kit, please add 2-hydroxyl-1-ethanethiol to Buffer cRL1 (10ul 2-hydroxyl-1-ethanethiol per 1mL Buffer cRL1). Buffer cRL1 can be stored at 4℃ for 1 month after adding 2-hydroxyl-1-ethanethiol. If the extracted RNA is not used to close full length cDNA, but only used for other downstream operations such as qPCR or sequencing analysis, it is not necessary to add 2-hydroxy-1-ethanethiol, and the result will not be affected.
• Before using the kit, please add anhydrous ethanol to buffer cRL2 and Buffer RW2 referring to the label on the reagent bottle for the dosage.
• The volume of elution should be no less than 20ul, otherwise it will influence RNA yield.
• Please check if there is any precipitate in Buffer cRL1 and Buffer RW1. If the precipitate can be seen after storage at low temperature, the solution should be placed at room temperature (15-25℃) or 37℃ for a period of time. Use the solution after dissolving and blending.